Monoclonal Antibodies - GLI1 Mouse Monoclonal Antibody

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GLI1 Mouse Monoclonal Antibody

Catalog #：EAB21207

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB21207-100UL	In Stock	100ul	$388.50

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email us at info@ebiocell.com

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	EbioCell	Phone：(540) 808-3925
	Imperial Business Park 4819 Emperor Boulevard,	E-mail：order@ebiocell.com
Canada	Suite 408 Durham, NC 27703, USA	Web：www.ebiocell.com

Product Information
Applications	WB, IHC-P, IF/ICC
Species Reactivity	Human, Mouse, Rat, Pig
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:5000-20000　 IHC-P=1:100-500　 IF/ICC=1:200-1000
MW (kDa)	118
Conjugate	Unconjugate
Specificity	GLI1 Mouse Monoclonal Antibody detects endogenous levels of GLI1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	GLI1; GLI; Zinc finger protein GLI1
Gene Aliases	GLI1
UniProt ID	P08151
Entrez Gene ID	2735

Product Description

GLI1 (GLI family zinc finger 1), also known as Glioma-associated oncogene or oncogene GLI, is a 1,106 amino acid protein that localizes to both the cytoplasm and nucleus, and belongs to the GLI C2H2-type zinc-finger protein family. GLI1 acts as a transcriptional activator and is thought to play a role in craniofacial development. GLI-1 exists as two alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 12q13.3.

Product Image Gallery
Western blot analysis of extracts on different cells, using GLI1 Mouse Monoclonal Antibody (EAB21207) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution. Lane1: MCF7 Lane2: HEK293 Lane3: A549 Predicted band size: 118 kDa; Observed band size: 150 kDa

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol