Monoclonal Antibodies - CD45 Mouse Monoclonal Antibody

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CD45 Mouse Monoclonal Antibody

Catalog #：EAB11035

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB11035-30UL	In Stock	30ul	€115.70
EAB11035-100UL	In Stock	100ul	€258.70
EAB11035-200UL	In Stock	200ul	€440.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	SBT BioChem Srl	Phone：+39 05 7156 8147
	Via Ponte di Bagnolo, 811 Bologna,	E-mail：info@sbtbiochem.it
Italy	Italy	Web：www.sbtbiochem.it

Product Information
Applications	WB, IHC-P, IF/ICC
Species Reactivity	Human
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-300　 IF/ICC=1:50-300
MW (kDa)	147
Conjugate	Unconjugate
Specificity	CD45 Mouse Monoclonal Antibody detects endogenous levels of CD45 fusion protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	B220; CD45; CD45R; GP180; L-CA; LCA; loc; Ly-5; LY5; Lyt-4; PTPRC; RT7; T200
Gene Aliases	PTPRC
UniProt ID	P08575
Entrez Gene ID	5788

Product Description

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains.

Product Image Gallery
Western blot analysis of extracts on Hela cells, using CD45 Mouse Monoclonal Antibody (EAB11035) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol