Antibiotics - Puromycin dihydrochloride (CAS: 58-58-2)

Location：Home > Biochemicals > Antibiotics

Puromycin dihydrochloride (CAS: 58-58-2)

Catalog #：EBC51170

Datasheet

Puromycin dihydrochloride
(CAS: 58-58-2)

SKU-Pack Size	Availability	Size	Price
EBC51170-1ML	In Stock	1mL(10mg/ml)	€50.70
EBC51170-25MG	In Stock	25mg	€76.70
EBC51170-100MG	In Stock	100mg	€284.70

If you want to get a larger packsize, Please contact your local distributor or
email us atinfo@ebiocell.com

Please Select The Country You Are In To Find Your Local Distributor.

	SBT BioChem Srl	Phone：+39 05 7156 8147
	Via Ponte di Bagnolo, 811 Bologna,	E-mail：info@sbtbiochem.it
Italy	Italy	Web：www.sbtbiochem.it

Product Information

Synonym(s)

CL13900 dihydrochloride; Stylomycin hydrochloride; 嘌呤霉素;

Chemical Name

(2S)-2-amino-N-[(2S,3S,4R,5R)-5-[6-(dimethylamino)purin-9-yl]-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]-3-(4-methoxyphenyl)propanamide;dihydrochloride

Application

Puromycin dihydrochloride is an antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene

CAS Number

58-58-2

Purity

≥98.0%

Molecular Weight

544.43

Molecular Formula

C₂₂H₂₉N₇O₅•2HCl

SMILES

CN(C)C1=NC=NC2=C1N=CN2[C@H]3[C@@H]([C@@H]([C@H](O3)CO)NC(=O)[C@@H](CC4=CC=C(C=C4)OC)N)O.Cl.Cl

Target & IC50

Aminoglycoside

Solubility

DMSO: 50 mg/mL (20 mM)
Water: 50 mg/mL (20 mM)

Preparing Stock Solutions

ConcentrationSolventMass	1 mg	5 mg	10 mg
1 mM	1.8368 ml	9.1839 ml	18.3678 ml
5 mM	0.3674 ml	1.8368 ml	3.6736 ml
10 mM	0.1837 ml	0.9184 ml	1.8368 ml
50 mM	0.0367 ml	0.1837 ml	0.3674 ml

Shipping

Gel Pack

Storage

Store at -20°C

Research Use

For Research Use Only. Not Intended for Diagnostic Or Therapeutic Use.

Product Description

Puromycin dihydrochloride, also known as Stylomycin hydrochloride, is an aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene, whose product, puromycin-N-acetyl-transferase, inactivates puromycin via acetylation. Puromycin dihydrochloride has been cell culture tested and is recommended for selection of stably transfected cells following transfection with Santa Cruz Biotechnology shRNA or Lentiviral particles. Puromycin dihydrochloride is an antibiotic substance produced by the soil actinomycete Streptomyces alboniger which induces apoptosis in cells by interfering with RNA function, leading to inhibition of protein synthesis. Puromycin dihydrochloride is thought to act as an acyl-tRNA analogue causing premature chain termination. Toxic to both eukaryotic and prokaryotic cells, Puromycin dihydrochloride has also been shown to arrest cells in G2/M phase.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol