Monoclonal Antibodies - KAP1/TIF1β Mouse Monoclonal Antibody

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KAP1/TIF1β Mouse Monoclonal Antibody

Catalog #：EAB21231

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB21231-100UL	In Stock	100ul	￡251.90

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	CUlabSciences	Phone：+44 (0) 1232 330008
	Cambridge House St Thomas' Place Ely,	E-mail：sales@culabsciences.co.uk
United Kingdom	Cambridge CB27 9RD UK	Web：www.culabsciences.co.uk

Product Information
Applications	WB, IP, IHC-P, IF/ICC
Species Reactivity	Human
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IP=1:50-200 IHC-P=1:100-500　 IF/ICC=1:50-300
MW (kDa)	89
Conjugate	Unconjugate
Specificity	KAP1/TIF1β Mouse Monoclonal Antibody detects endogenous levels of KAP1/TIF1β protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	KAP-1; KAP1; KRIP-1; Krip1; MommeD9; PPP1R157; RNF96; TF1B; TIF1B; Tif1beta; TRIM28
Gene Aliases	TRIM28
UniProt ID	Q13263
Entrez Gene ID	10155

Product Description

TIF1β, for transcriptional intermediary factor 1-beta, also designated KAP1(for KRAB-associated protein 1), TF1β and TRIM28 (for tripartif motif-containing 28), is a member of the tripartif motif family characterized by three zinc-binding domains, a RING finger, B-boxes and a coiled-coil domain. Like TIF1α, TIF1β contains both a Cys/His PHD (plant homeodomain) finger and bromodomain that form a cooperative unit required for transcriptional repression. TIF1β mediates transcriptional control by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors and by binding DNA through its zinc finger. The human TIF1β gene maps to human chromosome 19q13.4 and encodes an 835 amino acid nuclear protein.

Product Image Gallery

Western blot analysis of extracts on different cells, using KAP1/TIF1β Mouse Monoclonal Antibody (EAB21231) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Lane1: 293T
Lane2: HepG2

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues, using KAP1/TIF1β Mouse Monoclonal Antibody (EAB21231) at 1:400 dilution.

Immunofluorescence analysis of HepG2 cells, using KAP1/TIF1β Mouse Monoclonal Antibody (EAB21231) at 1:100 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol