Polyclonal Antibodies - Phospho-Smad4 (Thr276) Rabbit Polyclonal Antibody

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Phospho-Smad4 (Thr276) Rabbit Polyclonal Antibody

Catalog #：EAB10055

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB10055-30UL	In Stock	30ul	€115.70
EAB10055-100UL	In Stock	100ul	€297.70
EAB10055-200UL	In Stock	200ul	€479.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	OlaSciences	Phone：+33 (0) 9 77 4001 556
	87, rue des Suisses 65000 Nanterre,	E-mail：ola@olasciences.com
France	France	Web：www.olasciences.com

Product Information
Applications	WB
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000
MW (kDa)	60
Conjugate	Unconjugate
Specificity	Phospho-Smad4 (Thr276) Rabbit Polyclonal Antibody detects endogenous levels of Smad4 protein only when phosphorylated at Thr276.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	SMAD family member 4; JIP; DPC4; MADH4; MYHRS; SMAD4
Gene Aliases	SMAD4
UniProt ID	Q13485
Entrez Gene ID	4089

Product Description

Smad4 also known as DPC4 is a member of the Smad family of signal transduction proteins. Smad4 plays a crucial role as a central mediator in the transforming growth factor-beta (TGFβ) and bone morphogenetic protein (BMP) signaling pathways, which are essential for regulating cellular processes such as proliferation, differentiation, and apoptosis. By interacting with other Smad family members, Smad4 facilitates transcriptional regulation of target genes, thereby influencing developmental processes and tissue homeostasis.

Product Image Gallery
Western blot analysis of extracts on Hela cells, using Phospho-Smad4 (Thr276) Rabbit Polyclonal Antibody (EAB10055) at 1:1000 dilution. The image on the left is blocked with the phospho peptide.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol