SKU-Pack Size | Availability | Size | Price | |
EAB13798-30UL | In Stock | 30ul | €115.70 | |
EAB13798-100UL | In Stock | 100ul | €258.70 | |
EAB13798-200UL | In Stock | 200ul | €440.70 |
Please select the country you are in to find your local distributor. |
Dianost | Phone£º+49 (0) 40 65472-053 | |
Bullenkoppel 11£¬ 23896 Poggensee, | E-mail£ºola@olasciences.com | |
Germany | Germany | Web£ºwww.olasciences.com |
Product Information | |
Applications | WB, IHC-P |
Species Reactivity | Human |
Host / Isotype | Rabbit IgG |
Clonality | Polyclonal |
Applications Dilutions | WB=1:1000-5000¡¡ IHC-P=1:100-500 |
MW (kDa) | 73 |
Conjugate | Unconjugate |
Specificity | c-Raf Rabbit Polyclonal Antibody detects endogenous levels of c-Raf protein. |
Purification | Affinity purification |
Concentration | 1mg/ml |
Format | Liquid |
Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
Shipping | Gel Pack |
Storage | Store at -20¡ãC least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4¡ãC for 1-2 weeks. |
Research Use | For Research Use Only. Not Intended for Diagnostic or Therapeutic Use. |
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
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Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
Product Bioinformatics | |
Synonym(s) | CMD1NN; CRAF; NS5; Raf-1; c-Raf |
Gene Aliases | RAF1 |
UniProt ID | |
Entrez Gene ID |
Product Description | |
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. |
Product Image Gallery | |
Western blot analysis of extracts on Hela cells, using c-Raf/Raf1 Rabbit Polyclonal Antibody (EAB13798) at 1:2000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue, using c-Raf/Raf1 Rabbit Polyclonal Antibody (EAB13798) at 1:200 dilution.
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Specific Protocols | |