Polyclonal Antibodies - Phospho-p47phox (Ser359) Rabbit Polyclonal Antibody

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Phospho-p47phox (Ser359) Rabbit Polyclonal Antibody

Catalog #：EAB13495

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB13495-30UL	In Stock	30ul	€141.70
EAB13495-100UL	In Stock	100ul	€297.70
EAB13495-200UL	In Stock	200ul	€479.70

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email us at info@ebiocell.com

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	OlaSciences	Phone：+33 (0) 9 77 4001 556
	87, rue des Suisses 65000 Nanterre,	E-mail：ola@olasciences.com
France	France	Web：www.olasciences.com

Product Information
Applications	WB, IHC-P, IF/ICC, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-300　 IF/ICC=1:50-300
MW (kDa)	45
Conjugate	Unconjugate
Specificity	Phospho-p47phox (Ser359) Rabbit Polyclonal Antibody detects endogenous levels of p47phox only when phosphorylated at Ser359.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Neutrophil cytosol factor 1; CGD1; NCF1A; NOXO2; p47phox; SH3PXD1A; NCF-47K; NCF1; p47-phox
Gene Aliases	NCF1
UniProt ID	P14598
Entrez Gene ID	653361

Product Description

p47phox (also designated NCF1, NOXO2, SH3PXD1A or p47-phox) is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. During activation of the NADPH oxidase, p47phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 domain of p67-phox has been shown to interact with the proline-rich domain of p47phox, suggesting that p47phox may faciliate the transport of p67-phox to the membrane.

Product Image Gallery

Western blot analysis of extracts on Hela cells treated with Nocodazole, using Phospho-p47phox (Ser359) Rabbit Polyclonal Antibody (EAB13495) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue, using Phospho-p47phox (Ser359) Rabbit Polyclonal Antibody (EAB13495) at 1:100 dilution.

Immunofluorescence analysis of Hela cell, using Phospho-p47phox (Ser359) Rabbit Polyclonal Antibody (EAB13495) at 1:200 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol