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Location：Home > Antibodies > Primary Antibodies > Monoclonal Antibodies
DNA-PKcs Mouse Monoclonal Antibody
Catalog #：EAB22355
Datasheet
  SKU-Pack Size Availability Size Price
EAB22355-100UL In Stock 100ul ￥2290.00
If you want to get a larger packsize, Please contact your local distributor or
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2 / F, 128 malianwa North Road, Haidian District, E-mail：   mrkbio@163.com 
China Beijing China Website： www.mrkbio.com
Product Information
Applications WB, IP, IHC-P, IF/ICC
Species Reactivity Human
Host / Isotype Mouse IgG1
Clonality Monoclonal
Applications Dilutions WB=1:500-2000　
IP=1:20-200　
IHC-P=1:50-300　
IF/ICC=1:50-300　
MW (kDa) 450
Conjugate Unconjugate
Specificity DNA-PKcs Mouse Monoclonal Antibody detects endogenous levels of DNA-PKcs protein.
Purification Affinity purification
Concentration 1mg/ml
Format Liquid
Formulation In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping Gel Pack
Storage Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key

WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key

H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected
Product Bioinformatics
Synonym(s) DNA-PKcs; DNAPDcs; DNAPK; DNPK1; DOXNPH; dxnph; HYRC; HYRC1; IMD26; p350; p460; PRKDC; scid; slip; XRCC7
Gene Aliases PRKDC
UniProt ID

P78527
Entrez Gene ID

5591
Product Description

DNA-PKCS (also designated PRKDC, HYRC or DNA-PK catalytic subunit (DNPK1) ). DNA-PK is a heterotrimeric DNA binding enzyme that is composed of a large subunit, DNA-PKCS, and two smaller subunits collectively known as Ku. The loss of DNA-PK leads to defects in DSB repair and V(D)J recombination. FRAP can autophosphorylate on serine and bind to rapamycin/FKBP. FRAP is also an upstream regulator of S6 kinase and has been implicated in the regulation of p27 and p21 expression.
Product Image Gallery
Western blot analysis of extracts on different cells, using DNA-PKcs Mouse Monoclonal Antibody (EAB22355) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Lane1: A549
Lane2: K562
Immunohistochemical analysis of paraffin-embedded Breast cancer tissue, using DNA-PKcs Mouse Monoclonal Antibody (EAB22355) at 1:200 dilution.
Immunocytochemistry stain of Hela cell, using DNA-PKcs Mouse Monoclonal Antibody (EAB22355) at 1:100 dilution.
Specific Protocols
>> Western Blotting Protocol >> Immunoprecipitation Protocol
>> Immunohistochemistry Protocol >> Immunofluorescence Protocol
>> Immunocytochemistry Protocol >> Flow Cytometry Protocol
>> ChIP Protocol >> ELISA Protocol
>> HPLC Protocol >> PCR Protocol
For Research Use Only, Not For Diagnostic Or Therapeutic Procedures.
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