Monoclonal Antibodies - DNA-PKcs Mouse Monoclonal Antibody

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DNA-PKcs Mouse Monoclonal Antibody

Catalog #：EAB22355

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB22355-100UL	In Stock	100ul	$343.50

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	EbioCell	Phone：(540) 808-3925
	Imperial Business Park 4819 Emperor Boulevard,	E-mail：order@ebiocell.com
United States	Suite 408 Durham, NC 27703, USA	Web：www.ebiocell.com

Product Information
Applications	WB, IP, IHC-P, IF/ICC
Species Reactivity	Human
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IP=1:20-200　 IHC-P=1:50-300　 IF/ICC=1:50-300
MW (kDa)	469
Conjugate	Unconjugate
Specificity	DNA-PKcs Mouse Monoclonal Antibody detects endogenous levels of DNA-PKcs protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	DNA-dependent protein kinase catalytic subunit; DNA-PKcs; HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; S473K; XRCC7; DNAPKc; DNA-PKC; PRKDC
Gene Aliases	PRKDC
UniProt ID	P78527
Entrez Gene ID	5591

Product Description

DNA-PKcs also known as XRCC7, DNAPK, PRKDC is a critical component of the DNA-dependent protein kinase complex, DNA-PKcs plays a crucial role in the repair of DNA double-strand breaks (DSBs) and is essential for V(D)J recombination, a process vital for the development of immune diversity. Located in the nucleus, DNA-PKcs forms a heterotrimeric complex with Ku proteins, which are responsible for recognizing and binding to DSBs, thereby facilitating repair processes. Proper functioning of DNA-PKcs is critical, as defects can lead to severe consequences, including increased sensitivity to ionizing radiation and impaired DNA repair mechanisms, which are associated with various genetic disorders and cancers.

Product Image Gallery

Western blot analysis of extracts on A549, Hela, K562 cells, using DNA-PKcs Mouse Monoclonal Antibody (EAB22355) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Predicted band size: 469 kDa; Observed band size: 450 kDa

Immunohistochemical analysis of paraffin-embedded Human Breast cancer tissue, using DNA-PKcs Mouse Monoclonal Antibody (EAB22355) at 1:200 dilution.

Immunocytochemistry stain of Hela cell, using DNA-PKcs Mouse Monoclonal Antibody (EAB22355) at 1:100 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol