Monoclonal Antibodies - DNA-PKcs Mouse Monoclonal Antibody

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DNA-PKcs Mouse Monoclonal Antibody

Catalog #：EAB22355

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB22355-100UL	In Stock	100ul	€297.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

Please select the country you are in to find your local distributor.

	Cambiotech	Phone：+44 (0) 188 448801
	Lanwades Business Park,	E-mail：info@cambiotech.com
Ireland	Kobenhavn K, Ireland	Web：www.cambiotech.com

Product Information
Applications	WB, IP, IHC-P, IF/ICC
Species Reactivity	Human
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IP=1:20-200　 IHC-P=1:50-300　 IF/ICC=1:50-300
MW (kDa)	450
Conjugate	Unconjugate
Specificity	DNA-PKcs Mouse Monoclonal Antibody detects endogenous levels of DNA-PKcs protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	DNA-PKcs; DNAPDcs; DNAPK; DNPK1; DOXNPH; dxnph; HYRC; HYRC1; IMD26; p350; p460; PRKDC; scid; slip; XRCC7
Gene Aliases	PRKDC
UniProt ID	P78527
Entrez Gene ID	5591

Product Description

DNA-PKCS (also designated PRKDC, HYRC or DNA-PK catalytic subunit (DNPK1) ). DNA-PK is a heterotrimeric DNA binding enzyme that is composed of a large subunit, DNA-PKCS, and two smaller subunits collectively known as Ku. The loss of DNA-PK leads to defects in DSB repair and V(D)J recombination. FRAP can autophosphorylate on serine and bind to rapamycin/FKBP. FRAP is also an upstream regulator of S6 kinase and has been implicated in the regulation of p27 and p21 expression.

Product Image Gallery

Western blot analysis of extracts on different cells, using DNA-PKcs Mouse Monoclonal Antibody (EAB22355) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Lane1: A549
Lane2: K562

Immunohistochemical analysis of paraffin-embedded Breast cancer tissue, using DNA-PKcs Mouse Monoclonal Antibody (EAB22355) at 1:200 dilution.

Immunocytochemistry stain of Hela cell, using DNA-PKcs Mouse Monoclonal Antibody (EAB22355) at 1:100 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol