EAAT4 also known as SLC1A6 plays a crucial role in the central nervous system by functioning as an aspartate/glutamate transporter, predominantly expressed in the cerebellum. EAAT4 is essential for the reuptake of excitatory neurotransmitters L-glutamate and D-aspartate, which helps terminate the postsynaptic action of glutamate and prevents excitotoxicity—a condition that can lead to neuronal damage due to excessive accumulation of these neurotransmitters. The high affinity of EAAT4 for L-aspartate and L-glutamate underscores its importance in maintaining synaptic balance and protecting neurons from potential excitotoxic effects. Additionally, EAAT4′s transport activity is vital for proper cerebellar function, influencing motor coordination and cognitive processes.