Recombinant Antibodies - Phospho-Smad1 (Ser463/Ser465)/Smad5 (Ser463/Ser465)/Smad9 (Ser465/Ser467) Rabbit Monoclonal Antibody

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Phospho-Smad1 (Ser463/Ser465)/Smad5 (Ser463/Ser465)/Smad9 (Ser465/Ser467) Rabbit Monoclonal Antibody

Catalog #：EAB22123

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB22123-100UL	In Stock	100ul	$343.50

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	EbioCell	Phone：(540) 808-3925
	Imperial Business Park 4819 Emperor Boulevard,	E-mail：order@ebiocell.com
United States	Suite 408 Durham, NC 27703, USA	Web：www.ebiocell.com

Product Information
Applications	WB
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000
MW (kDa)	52
Conjugate	Unconjugate
Specificity	Phospho-Smad1 (Ser463/Ser465)/Smad5 (Ser463/Ser465)/Smad9 (Ser465/Ser467) Rabbit Monoclonal Antibody detects endogenous levels of Smad1 and Smad5 only when phosphorylated at Ser463/Ser465 and Smad9 when phosphorylated at Ser465/467.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	BSP-1; BSP1; Dwf-C; DWFC; JV4-1; JV41; JV5-1; Mad1; MADH1; MADH5; MADH6; Madh8; MADH9; MADR1; Mlp1; Msmad5; MusMLP; PPH2; SMAD1; SMAD5; SMAD8; SMAD8/9; SMAD8A; SMAD8B; SMAD9
Gene Aliases	SMAD1/SMAD5/SMAD9
UniProt ID	Q15797, Q99717, O15198
Entrez Gene ID	4086, 4090, 4093

Product Description

Smad1 also known as MADH1, JV4-1, MADR1 is an intracellular signal transducer and transcriptional modulator involved in the Bone Morphogenetic Protein (BMP) pathway, part of the Transforming Growth Factor-beta (TGF-β) superfamily. Upon activation by BMP receptors, Smad1 is phosphorylated and forms a complex with Smad4, translocating from the cytoplasm to the nucleus. This nuclear localization is crucial because Smad1 regulates the expression of target genes that control cell proliferation, differentiation, and apoptosis. Smad1 function is essential for embryonic development, tissue homeostasis, and bone formation. Dysregulation of Smad1 signaling has been implicated in various diseases, including cancer and fibrosis.

Product Image Gallery
Western blot analysis of extracts on Hela, MCF7, HepG2 cells, using Phospho-Smad1 (Ser463/Ser465)/Smad5 (Ser463/Ser465)/Smad9 (Ser465/Ser467) Rabbit Monoclonal Antibody (EAB22123) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol