P23921

Ribonucleotide reductase is essential for the production and maintenance of the level of deoxyribonucleoside triphosphates (dNTP′s) required for DNA synthesis. It is an enzymatic complex consisting of two nonidentical subunits, R1 and R2, which are inactive separately. R1, the larger subunit, contains allosteric regulatory sites in a human breast carcinoma cell line. R2 is the limiting factor of the catalytic activity of the ribonucleotide reductase enzymatic complex. R2 expression is strictly correlated to the S-phase of the cell cycle, whereas R1 remains constant throughout all phases of the cell cycle. Ribonucleotide reductase appears to be specifically involved in nucleotide excision repair, since both the R1 and R2 subunits are induced in response to UV light in a dose-dependent manner.