Recombinant Antibodies - Phospho-Nucleolin (Thr84) Rabbit Monoclonal Antibody

Phospho-Nucleolin (Thr84) Rabbit Monoclonal Antibody

Catalog #：EAB22035

	SKU-Pack Size	Availability	Size	Price
	EAB22035-100UL	In Stock	100ul	￡284.90

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	CUlabSciences	Phone：+44 (0) 1232 330008
	Cambridge House St Thomas' Place Ely,	E-mail：sales@culabsciences.co.uk
United Kingdom	Cambridge CB27 9RD UK	Web：www.culabsciences.co.uk

Product Information
Applications	WB
Species Reactivity	Human
Host / Isotype	Rabbit IgG
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000
MW (kDa)	77
Conjugate	Unconjugate
Specificity	Phospho-Nucleolin (Thr84) Rabbit Monoclonal Antibody detects endogenous levels of Nucleolin only when phosphorylated at Thr84.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	C23; NCL; Nucl; Nucleolin; nucleolin-like protein; Protein C23; D0Nds28; D1Nds28; Nuc
Gene Aliases	NCL
UniProt ID	P19338
Entrez Gene ID	4691

Product Description
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

Product Image Gallery
Western blot analysis of extracts on HepG2, MCF7 cells, using Phospho-Nucleolin (Thr84) Rabbit Monoclonal Antibody (EAB22035) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution. Predicted band size: 77 kDa; Observed band size: 100 kDa

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol