Recombinant Antibodies - NCK2 Rabbit Monoclonal Antibody

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NCK2 Rabbit Monoclonal Antibody

Catalog #：EAB22152

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB22152-100UL	In Stock	100ul	€297.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	Dianost	Phone：+49 (0) 40 65472-053
	Bullenkoppel 11， 23896 Poggensee,	E-mail：ola@olasciences.com
Germany	Germany	Web：www.olasciences.com

Product Information
Applications	WB, IHC-P, IF/ICC
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-200　 IF/ICC=1:50-200
MW (kDa)	43
Conjugate	Unconjugate
Specificity	NCK2 Rabbit Monoclonal Antibody detects endogenous levels of NCK2 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	NCK adaptor protein 2; GRB4; NCKbeta; NCK2
Gene Aliases	NCK2
UniProt ID	O43639
Entrez Gene ID	8440

Product Description

NCK2 also known as NCKbeta, GRB4 is a member of the NCK family of adaptor proteins. NCK2 plays a crucial role in cellular signaling as a member of the NCK family of SH2/SH3 adaptor proteins, which also includes NCK1. These proteins are essential for coupling tyrosine kinase signaling pathways, such as those activated by epidermal growth factor (EGF) and platelet-derived growth factor (PDGF), to downstream signaling proteins. NCK2 binds to human PDGFR-β at tyrosine 1009, and overexpression has been shown to inhibit PDGF-induced membrane ruffling and lamellipod formation, thereby regulating cell motility and morphology. Additionally, the SH2 domain of NCK2 has been implicated in the inhibition of EGF- and PDGF-induced DNA synthesis, highlighting a role in controlling cell proliferation.

Product Image Gallery
Western blot analysis of extracts on MCF7, Mouse brain cells, using NCK2 Rabbit Monoclonal Antibody (EAB22152) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol