Recombinant Antibodies - DNA Ligase I/LIG1 Rabbit Monoclonal Antibody

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DNA Ligase I/LIG1 Rabbit Monoclonal Antibody

Catalog #：EAB21984

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB21984-100UL	In Stock	100ul	€297.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

Please select the country you are in to find your local distributor.

	Cambiotech	Phone：+44 (0) 188 448801
	Lanwades Business Park,	E-mail：info@cambiotech.com
Ireland	Kobenhavn K, Ireland	Web：www.cambiotech.com

Product Information
Applications	WB, IHC-P, IF/ICC
Species Reactivity	Human, Rat
Host / Isotype	Rabbit IgG
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-200　 IF/ICC=1:50-200
MW (kDa)	102
Conjugate	Unconjugate
Specificity	DNA Ligase I/LIG1 Rabbit Monoclonal Antibody detects endogenous levels of DNA Ligase I/LIG1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	LIG1; DNA ligase 1; DNA ligase I; ligase1; ligaseI; ATP-dependent; Lig-1; LIGI; Lig-I; DNLI1
Gene Aliases	LIG1
UniProt ID	P18858
Entrez Gene ID	3978

Product Description

DNA Ligase I also known as LIG1 is a member of the ATP-dependent DNA ligase protein family. DNA Ligase I plays a vital role in DNA replication and repair by joining Okazaki fragments during lagging strand synthesis and sealing nicks in DNA during excision repair processes. DNA Ligase I is predominantly located in the nucleus, where DNA Ligase I activity is essential for proper DNA repair pathways, ensuring efficient repair of DNA breaks to prevent genomic instability. Notably, elevated levels of DNA Ligase I have been observed in various human tumors compared to benign tissues, highlighting DNA Ligase I potential as a biomarker for cancer progression and a target for therapeutic intervention. Additionally, DNA Ligase I antisense oligonucleotides have been shown to reduce tumor cell proliferation, suggesting a significant role in cancer biology.

Product Image Gallery
Western blot analysis of extracts on Jurkat cells, using DNA Ligase I/LIG1 Rabbit Monoclonal Antibody (EAB21984) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution. Predicted band size: 102 kDa; Observed band size: 140 kDa

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol