Polyclonal Antibodies - Eg5 Rabbit Polyclonal Antibody

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Eg5 Rabbit Polyclonal Antibody

Catalog #：EAB11758

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB11758-30UL	In Stock	30ul	$133.50
EAB11758-100UL	In Stock	100ul	$298.50
EAB11758-200UL	In Stock	200ul	$508.50

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email us at info@ebiocell.com

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	EbioCell	Phone：(540) 808-3925
	Imperial Business Park 4819 Emperor Boulevard,	E-mail：order@ebiocell.com
United States	Suite 408 Durham, NC 27703, USA	Web：www.ebiocell.com

Product Information
Applications	WB, IHC-P, IF/ICC, ELISA
Species Reactivity	Human, Mouse
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　　 IHC-P=1:50-300 IF/ICC=1:50-300
MW (kDa)	119
Conjugate	Unconjugate
Specificity	Eg5 Rabbit Polyclonal Antibody detects endogenous levels of Eg5 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Kinesin family member 11; EG5; HKSP; KNSL1; MCLMR; TRIP5; KIF11
Gene Aliases	KIF11
UniProt ID	P52732
Entrez Gene ID	3832

Product Description

Eg5 also known as TRIP5, KIF11 is a slow plus-end-directed microtubule-based motor of the BimC kinesin family that is essential for bipolar spindle formation during eukaryotic cell division. When the expression of Eg5 is blocked, centrosome migration halts and cells are arrested in mitosis with monoastral microtubule arrays. Eg5 is phosphorylated on serine during S phase and on both serine and Thr 927 during mitosis, which regulates the association of Eg5 with the spindle apparatus (probably during early prophase). Eg5 is also known to be a member of the thyroid receptor interacting protein (Trip) family, and interacts with the thyroid hormone receptor only in the presence of thyroid.

Product Image Gallery
Western blot analysis of extracts on HepG2, Jurkat, COLO205 cells, using Eg5 Rabbit Polyclonal Antibody (EAB11758) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol