Monoclonal Antibodies - 4E-BP1 Mouse Monoclonal Antibody

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4E-BP1 Mouse Monoclonal Antibody

Catalog #：EAB22001

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB22001-100UL	In Stock	100ul	$388.50

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email us at info@ebiocell.com

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	EbioCell	Phone：(540) 808-3925
	Imperial Business Park 4819 Emperor Boulevard,	E-mail：order@ebiocell.com
United States	Suite 408 Durham, NC 27703, USA	Web：www.ebiocell.com

Product Information
Applications	WB, IHC-P, IF/ICC, ELISA
Species Reactivity	Human, Mouse, Rat, Pig
Host / Isotype	Mouse IgG2b
Clonality	Monoclonal
Applications Dilutions	WB=1:1000-5000　 IHC-P=1:50-300 IF=1:50-300
MW (kDa)	13
Conjugate	Unconjugate
Specificity	4E-BP1 Mouse Monoclonal Antibody detects endogenous levels of 4E-BP1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	BP-1; 4EBP1; 4E-BP1; PHAS-I; EIF4EBP1
Gene Aliases	EIF4EBP1
UniProt ID	Q13541
Entrez Gene ID	1978

Product Description

4E-BP1 also known as EIF4EBP1 is one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Product Image Gallery
Western blot analysis of extracts on K562, HCT116 cells, using 4E-BP1 Mouse Monoclonal Antibody (EAB22001) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution. Predicted band size: 13 kDa; Observed band size: 15-20 kDa

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol