Monoclonal Antibodies - c-Myc Mouse Monoclonal Antibody

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c-Myc Mouse Monoclonal Antibody

Catalog #：EAB21315

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB21315-100UL	In Stock	100ul	$343.50

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	EbioCell	Phone：(540) 808-3925
	Imperial Business Park 4819 Emperor Boulevard,	E-mail：order@ebiocell.com
United States	Suite 408 Durham, NC 27703, USA	Web：www.ebiocell.com

Product Information
Applications	WB, IHC-P, IF/ICC, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse IgG2b
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500
MW (kDa)	50
Conjugate	Unconjugate
Specificity	c-Myc Mouse Monoclonal Antibody detects endogenous levels of c-Myc protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	MRTL; MYCC; c-Myc; bHLHe39
Gene Aliases	MYC
UniProt ID	P01106
Entrez Gene ID	4609

Product Description

c-Myc is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.

Product Image Gallery

Western blot analysis of extracts on different cells, using c-Myc Mouse Monoclonal Antibody (EAB21315) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Lane1: A549
Lane2: NIH/3T3

Predicted band size: 50 kDa; Observed band size: 50-65 kDa

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, using c-Myc Mouse Monoclonal Antibody (EAB21315) at 1:500 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol