SKU-Pack Size | Availability | Size | Price | |
EAB22359-100UL | In Stock | 100ul | $388.50 |
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EbioCell | Phone:(540) 808-3925 | |
Imperial Business Park 4819 Emperor Boulevard, | E-mail:order@ebiocell.com | |
United States | Suite 408 Durham, NC 27703, USA | Web:www.ebiocell.com |
Product Information | |
Applications | WB, IHC-P, IF/ICC, ELISA |
Species Reactivity | Human, Rat |
Host / Isotype | Mouse IgG1 |
Clonality | Monoclonal |
Applications Dilutions | WB=1:5000-20000 IHC-P=1:100-500 IF/ICC=1:100-500 |
MW (kDa) | 87 |
Conjugate | Unconjugate |
Specificity | CUL2 Mouse Monoclonal Antibody detects endogenous levels of CUL2 protein. |
Purification | Affinity purification |
Concentration | 1mg/ml |
Format | Liquid |
Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
Shipping | Gel Pack |
Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
Research Use | For Research Use Only. Not Intended for Diagnostic or Therapeutic Use. |
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
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Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
Product Bioinformatics | |
Synonym(s) | CUL2; Cullin-2; CUL-2; Cullin2 |
Gene Aliases | Cullin-2 |
UniProt ID | |
Entrez Gene ID |
Product Description | |
Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. |
Product Image Gallery | |
Western blot analysis of extracts on different cells, using CUL2 Mouse Monoclonal Antibody (EAB22359) at 1:10000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Lane1: HEK293 Lane2: PC3 |
Specific Protocols | |