SKU-Pack Size | Availability | Size | Price | |
EAB11719-30UL | In Stock | 30ul | ¥890.00 | |
EAB11719-100UL | In Stock | 100ul | ¥1990.00 | |
EAB11719-200UL | In Stock | 200ul | ¥3390.00 |
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北京美瑞克生物科技有限公司 | Phone: +86 010-62890160 / 13691184142(微信同号) | |
2 / F, 128 malianwa North Road, Haidian District, | E-mail: mrkbio@163.com | |
China | Beijing China | Website: www.mrkbio.com |
Product Information | |
Applications | WB, ELISA |
Species Reactivity | Human, Mouse |
Host / Isotype | Rabbit IgG |
Clonality | Polyclonal |
Applications Dilutions | WB=1:500-2000 |
MW (kDa) | 120 |
Conjugate | Unconjugate |
Specificity | BUBR1 Rabbit Polyclonal Antibody detects endogenous levels of BUBR1 protein. |
Purification | Affinity purification |
Concentration | 1mg/ml |
Format | Liquid |
Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
Shipping | Gel Pack |
Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
Research Use | For Research Use Only. Not Intended for Diagnostic or Therapeutic Use. |
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
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Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
Product Bioinformatics | |
Synonym(s) | Bub1A; BUB1B; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1 |
Gene Aliases | BUB1B |
UniProt ID | |
Entrez Gene ID |
Product Description | |
Human cells contain two related protein kinases, BUB1 and BUBR1, that appear to have evolved from a single ancestral BUB1 gene. Both kinases are concentrated near the surface of the kinetochore where they monitor kinetochore-microtubule interactions. BUB1 and BUBR1 bind to kinetochores and are postulated to be components of the mitotic checkpoint, which monitors kinetochore activities to determine if chromosomes have achieved alignment at the spindle equator. BUBR1 is essential for normal mitotic progression as it prevents cells from prematurely entering anaphase. BUB3 is a conserved component of the mitotic spindle assembly complex and is also involved with the essential spindle checkpoint pathway that operates during early embryogenesis. |
Product Image Gallery | |
Western blot analysis of extracts on different cells, using BUBR1 Rabbit Polyclonal Antibody (EAB11719) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Lane1: Hela Lane2: HepG2 Predicted band size: 120 kDa; Observed band size: 130 kDa |
Specific Protocols | |