MCAD also known as ACADM belongs to the acyl-CoA dehydrogenase family. MCAD plays a crucial role in the mitochondrial fatty acid β-oxidation pathway, specifically catalyzing the initial reaction in the breakdown of medium-chain acyl-CoAs ranging from C4 to C12. This enzymatic activity is vital for energy production, particularly during periods of fasting when the body relies on fat stores for fuel. Deficiencies in MCAD can lead to severe metabolic disorders, including fasting hypoglycemia and hepatic dysfunction, which can be life-threatening, especially in infants. Notably, MCAD exhibits 88% sequence identity with its porcine counterpart, highlighting evolutionary conservation and importance across species.