Recombinant Antibodies - Phospho-Smad2 (Ser250) Rabbit Monoclonal Antibody

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Phospho-Smad2 (Ser250) Rabbit Monoclonal Antibody

Catalog #：EAB21783

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB21783-100UL	In Stock	100ul	￡251.90

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	CUlabSciences	Phone：+44 (0) 1232 330008
	Cambridge House St Thomas' Place Ely,	E-mail：sales@culabsciences.co.uk
United Kingdom	Cambridge CB27 9RD UK	Web：www.culabsciences.co.uk

Product Information
Applications	WB, FC
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 FC=1:10-100
MW (kDa)	52
Conjugate	Unconjugate
Specificity	Phospho-Smad2 (Ser250) Rabbit Monoclonal Antibody detects endogenous levels of Smad2 only when phosphorylated at Ser250.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	SMAD family member 2; JV18; LDS6; CHTD8; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; SMAD2
Gene Aliases	SMAD2
UniProt ID	Q15796
Entrez Gene ID	4087

Product Description

Smad2 also known as MADR2 is a critical intracellular mediator in the Transforming Growth Factor-beta (TGF-β) signaling pathway. Smad2 plays a crucial role in the transforming growth factor-beta (TGF-β) signaling pathway, which is essential for regulating cellular processes such as proliferation, differentiation, and apoptosis. Smad2 is primarily located in the cytoplasm and nucleus, where Smad2 translocates upon phosphorylation in response to TGF-β signaling. This localization enables Smad2 to act as a transcription factor, regulating the expression of target genes that mediate the cellular response to TGF-β. Smad2′s ability to shuttle between the cytoplasm and nucleus underscores Smad2′s importance in cellular signaling and gene regulation, making Smad2 a key player in developmental processes and tissue homeostasis. Smad2 interacts with other Smad proteins, such as Smad3 and Smad4, to form complexes that facilitate the transcription of TGF-β target genes, further emphasizing Smad2′s role in the intricate network of cellular signaling pathways.

Product Image Gallery
Western blot analysis of extracts on Hela cells, using Phospho-Smad2 (Ser250) Rabbit Monoclonal Antibody (EAB21783) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution. Predicted band size: 52 kDa; Observed band size: 58 kDa

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol