SKU-Pack Size | Availability | Size | Price | |
EAB21552-100UL | In Stock | 100ul | ¥2290.00 |
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北京美瑞克生物科技有限公司 | Phone: +86 010-62890160 / 13691184142(微信同号) |
2 / F, 128 malianwa North Road, Haidian District, | E-mail: mrkbio@163.com | |
China | Beijing China | Website: www.mrkbio.com |
Product Information | |
Applications | WB, IP, IHC-P, IF/ICC, FC |
Species Reactivity | Human, Mouse, Rat |
Host / Isotype | Rabbit IgG |
Clonality | Monoclonal |
Applications Dilutions | WB=1:500-2000 IP=1:10-100 IHC-P=1:50-200 IF/ICC=1:50-200 FC=1:10-100 |
MW (kDa) | 56 |
Conjugate | Unconjugate |
Specificity | Akt1 Rabbit Monoclonal Antibody detects endogenous levels of Akt1 protein. |
Purification | Affinity purification |
Concentration | 1mg/ml |
Format | Liquid |
Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
Shipping | Gel Pack |
Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
Research Use | For Research Use Only. Not Intended for Diagnostic or Therapeutic Use. |
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
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Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
Product Bioinformatics | |
Synonym(s) | AKT; AKT1; CWS6; PKB; PKB-ALPHA; PKB/Akt; PKBalpha; PRKBA; RAC; RAC-ALPHA |
Gene Aliases | AKT1 |
UniProt ID | |
Entrez Gene ID |
Product Description | |
The Akt family, comprising Akt1, Akt2, and Akt3, also referred to as protein kinase B isoforms, plays a pivotal role in regulating critical cellular processes, including growth, metabolism, and survival. Akt1, the most extensively studied isoform, is central to anti-apoptotic signaling, translocating from the cytoplasm to the plasma membrane upon activation by upstream kinases such as PI3K and PDK1. This membrane localization allows phosphorylation at Thr308 and Ser473, modifications necessary for full activation and interaction with downstream targets like glycogen synthase kinase-3 beta (GSK-3β) and mammalian target of rapamycin (mTOR). These interactions influence diverse cellular responses, including glucose uptake, protein synthesis, and cell cycle progression. Akt2, predominantly expressed in insulin-responsive tissues, regulates glucose metabolism, while Akt3 is implicated in brain development and function. The precise regulation of these isoforms through phosphorylation and localization underscores their importance in maintaining cellular homeostasis. Dysregulation of Akt signaling is associated with various diseases, including cancer, diabetes, and neurodegenerative disorders. |
Product Image Gallery | |
![]() Western blot analysis of extracts on MCF7 cells, using Akt1 Rabbit Monoclonal Antibody (EAB21562) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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Specific Protocols | |