GSK-3β also known as GSK3beta, GSK3B is a serine/threonine kinase with a highly conserved structure, featuring a kinase domain essential for phosphorylating various substrates. GSK-3β′s structure includes an ATP-binding pocket and a catalytic core that enables interaction with other proteins, allowing regulation of multiple signaling pathways, including those involved in glycogen synthesis and cell cycle control. The structural integrity of GSK-3β is crucial for activity, as alterations in conformation can disrupt the ability to phosphorylate key substrates, leading to impaired cellular functions. GSK-3β′s kinase domain also contains regions responsible for recognizing specific phosphorylation sites on substrates, further influencing regulatory role in cellular processes. This structural precision is particularly important in maintaining normal cell functions and has been implicated in various diseases, including neurodegenerative disorders.