Recombinant Antibodies - Phospho-Smad5 (Ser463/465) Rabbit Monoclonal Antibody

Phospho-Smad5 (Ser463/465) Rabbit Monoclonal Antibody

Catalog #：EAB22601

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Product Information
Applications	WB, IHC-P, IF/ICC
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-200　 IF/ICC=1:50-200
MW (kDa)	52
Conjugate	Unconjugate
Specificity	Phospho-Smad5 (Ser463/465) Rabbit Monoclonal Antibody detects endogenous levels of Smad1 and Smad5 only when dually phosphorylated at Ser463 and Ser465.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Dwf-C; DWFC; JV5-1; MADH5; Msmad5; MusMLP; SMAD5
Gene Aliases	SMAD5
UniProt ID	Q99717
Entrez Gene ID	4090

Product Description
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.

Product Image Gallery
Western blot analysis of extracts on Mouse brain, Rat brain cells, using Phospho-Smad5 (Ser463/465) Rabbit Monoclonal Antibody (EAB22601) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol