SKU-Pack Size | Availability | Size | Price | |
EAB14576-30UL | In Stock | 30ul | €115.70 | |
EAB14576-100UL | In Stock | 100ul | €258.70 | |
EAB14576-200UL | In Stock | 200ul | €440.70 |
Please select the country you are in to find your local distributor. |
OlaSciences | Phone£º+33 (0) 9 77 4001 556 | |
87, rue des Suisses 65000 Nanterre, | E-mail£ºola@olasciences.com | |
France | France | Web£ºwww.olasciences.com |
Product Information | |
Applications | WB, IHC-P, IF/ICC, ELISA |
Species Reactivity | Human, Mouse, Rat |
Host / Isotype | Rabbit IgG |
Clonality | Polyclonal |
Applications Dilutions | WB=1:500-2000¡¡ IHC-P=1:50-300¡¡ IF/ICC=1:50-300¡¡ |
MW (kDa) | 147 |
Conjugate | Unconjugate |
Specificity | CD45 Rabbit Polyclonal Antibody detects endogenous levels of CD45 protein. |
Purification | Affinity purification |
Concentration | 1mg/ml |
Format | Liquid |
Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
Shipping | Gel Pack |
Storage | Store at -20¡ãC least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4¡ãC for 1-2 weeks. |
Research Use | For Research Use Only. Not Intended for Diagnostic or Therapeutic Use. |
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
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Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
Product Bioinformatics | |
Synonym(s) | B220; CD45; CD45R; GP180; L-CA; LCA; loc; Ly-5; LY5; Lyt-4; PTPRC; RT7; T200 |
Gene Aliases | PTPRC |
UniProt ID | |
Entrez Gene ID |
Product Description | |
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. |
Product Image Gallery | |
Western blot analysis of extracts on Jurkat cells, using CD45 Rabbit Polyclonal Antibody (EAB14576) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue, using CD45 Rabbit Polyclonal Antibody (EAB14576) at 1:100 dilution.
Immunofluorescence analysis of HuvEc cell, using CD45 Rabbit Polyclonal Antibody (EAB14576) at 1:100 dilution.
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Specific Protocols | |