Polyclonal Antibodies - Acetyl-Histone H2A.X (Lys5) Rabbit Polyclonal Antibody

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Acetyl-Histone H2A.X (Lys5) Rabbit Polyclonal Antibody

Catalog #：EAB10792

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB10792-100UL	In Stock	100ul	€297.70
EAB10792-200UL	In Stock	200ul	€479.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	SBT BioChem Srl	Phone：+39 05 7156 8147
	Via Ponte di Bagnolo, 811 Bologna,	E-mail：info@sbtbiochem.it
Italy	Italy	Web：www.sbtbiochem.it

Product Information
Applications	WB, IHC-P
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:100-500
MW (kDa)	15
Conjugate	Unconjugate
Specificity	Acetyl-Histone H2A.X (Lys5) Rabbit Polyclonal Antibody detects endogenous levels of of histone H2A.X only when acetylated at Lys5.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	AW228881; gammaH2ax; H2A.X; H2A/X; H2AFX; H2AX; Hist5-2ax; Histone H2A.X; Histone H2a/x; Histone H2AX
Gene Aliases	H2AFX
UniProt ID	P16104
Entrez Gene ID	3014

Product Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Product Image Gallery
Western blot analysis of extracts on Hela cells, untreated (-) or treated with TSA (1μM, 18 hr; +), using Acetyl-Histone H2A.X (Lys5) Rabbit Polyclonal Antibody (EAB10792) at 1:2000 dilution. Immunohistochemical analysis of paraffin-embedded Human breast caricnoma tissue, using Acetyl-Histone H2A.X (Lys5) Rabbit Polyclonal Antibody (EAB10792) at 1:500 dilution

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol