Polyclonal Antibodies - GEF-H1 Rabbit Polyclonal Antibody

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GEF-H1 Rabbit Polyclonal Antibody

Catalog #：EAB10770

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB10770-30UL	In Stock	30ul	€115.70
EAB10770-100UL	In Stock	100ul	€258.70
EAB10770-200UL	In Stock	200ul	€440.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	Betaclonal	Phone：+34 (0)91 471 6763
	29/1 Lomonosovsky Pr,	E-mail：tech@betaclonal.com
Spain	Madrid, Spain	Web：www.betaclonal.com

Product Information
Applications	WB, IHC-P, IF, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-300 IF=1:50-300
MW (kDa)	112
Conjugate	Unconjugate
Specificity	GEF-H1 Rabbit Polyclonal Antibody detects endogenous levels of GEF-H1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Rho/Rac guanine nucleotide exchange factor 2; GEF; Lfc; P40; GEFH1; LFP40; GEF-H1; NEDMHM; ARHGEF2
Gene Aliases	ARHGEF2
UniProt ID	Q92974
Entrez Gene ID	9181

Product Description

GEF-H1 also known as Lfc, ARHGEF2 is a Rho GEF that localizes to microtubules and regulates Rho activity in response to microtubule destabilization. Loss of interaction between GEF-H1 and microtubules leads to activation of Rho. Phosphorylation of GEF-H1 at Ser886, a site located in the 14-3-3 binding motif, has been implicated in recruitment of 14-3-3 and GEF-H1 to microtubules, and in the regulation of RhoA activity in response to mitotic kinases during cytokinesis.GEF-H1 has also been shown to localize to tight junctions and modulate polarized cell permeability. GEF-H1 is inactivated by binding to cingulin at epithelial tight junctions, inactivating RhoA and leading to G1/S arrest.

Product Image Gallery
Western blot analysis of extracts on HT29, Raw264.7 cells, using GEF-H1 Rabbit Polyclonal Antibody (EAB10770) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol