SKU-Pack Size | Availability | Size | Price | |
EAB10523-30UL | In Stock | 30ul | €115.70 | |
EAB10523-100UL | In Stock | 100ul | €258.70 | |
EAB10523-200UL | In Stock | 200ul | €440.70 |
Please select the country you are in to find your local distributor. |
SBT BioChem Srl | Phone£º+39 05 7156 8147 | |
Via Ponte di Bagnolo, 811 Bologna, | E-mail£ºinfo@sbtbiochem.it | |
Italy | Italy | Web£ºwww.sbtbiochem.it |
Product Information | |
Applications | WB, IHC-P |
Species Reactivity | Human, Mouse |
Host / Isotype | Rabbit IgG |
Clonality | Polyclonal |
Applications Dilutions | WB=1:500-2000¡¡ IHC-P=1:50-300 |
MW (kDa) | 36 |
Conjugate | Unconjugate |
Specificity | c-Jun Rabbit Polyclonal Antibody detects endogenous levels of c-Jun protein. |
Purification | Affinity purification |
Concentration | 1mg/ml |
Format | Liquid |
Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
Shipping | Gel Pack |
Storage | Store at -20¡ãC least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4¡ãC for 1-2 weeks. |
Research Use | For Research Use Only. Not Intended for Diagnostic or Therapeutic Use. |
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
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Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
Product Bioinformatics | |
Synonym(s) | AP-1; AP1; c-Jun; JUN; Junc; Rjg-9 |
Gene Aliases | JUN |
UniProt ID | |
Entrez Gene ID |
Product Description | |
The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, while Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. An inhibitor of Fos/Jun function, termed IP-1, associates with Fos and Jun and is inactivated upon phosphorylation induced by the cAMP-dependent protein kinase A (PKA). |
Product Image Gallery | |
Western blot analysis of extracts on 293T, Hela, NIH/3T3 cells, using c-Jun Rabbit Polyclonal Antibody (EAB10523) at 1:2000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Lane1: Hela Lane2: 293T Lane3: NIH/3T3 Predicted band size: 36 kDa; Observed band size: 43 kDa Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue, using c-Jun Rabbit Polyclonal Antibody (EAB10523) at 1:200 dilution.
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Specific Protocols | |