Monoclonal Antibodies - c-Fos Mouse Monoclonal Antibody

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c-Fos Mouse Monoclonal Antibody

Catalog #：EAB10521

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB10521-30UL	In Stock	30ul	€115.70
EAB10521-100UL	In Stock	100ul	€258.70
EAB10521-200UL	In Stock	200ul	€440.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

Please select the country you are in to find your local distributor.

	Betaclonal	Phone：+34 (0)91 471 6763
	29/1 Lomonosovsky Pr,	E-mail：tech@betaclonal.com
Spain	Madrid, Spain	Web：www.betaclonal.com

Product Information
Applications	WB, IP, IHC-P, IF
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:1000-5000　 IP=1:100-500 IHC-P=1:100-500　 IF=1:50-300
MW (kDa)	41
Conjugate	Unconjugate
Specificity	c-Fos Mouse Monoclonal Antibody detects endogenous levels of c-Fos protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	AP-1; C-FOS; cFos; D12Rfj1; FOS; G0S7; p55
Gene Aliases	FOS
UniProt ID	P01100
Entrez Gene ID	2353

Product Description

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. c-Fos is a proto-oncogene that is the human homolog of the retroviral oncogene v-fos. c-fos is 380 amino acid protein with a basic leucine zipper region for dimerisation and DNA-binding and a transactivation domain at C-terminus.

Product Image Gallery

Western blot analysis of extracts on different cells, using c-Fos Mouse Monoclonal Antibody (EAB10521) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Lane1: Hela
Lane2: 293T
Lane3: Mouse Brain
Lane4: Rat Brain

Predicted band size: 41 kDa; Observed band size: 62 kDa

Immunohistochemical analysis of paraffin-embedded Human breast caricnoma tissue, using c-Fos Mouse Monoclonal Antibody (EAB10521) at 1:200 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol