Monoclonal Antibodies - PCNA Mouse Monoclonal Antibody

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PCNA Mouse Monoclonal Antibody

Catalog #：EAB10347

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB10347-30UL	In Stock	30ul	€115.70
EAB10347-100UL	In Stock	100ul	€258.70
EAB10347-200UL	In Stock	200ul	€440.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	Dianost	Phone：+49 (0) 40 65472-053
	Bullenkoppel 11， 23896 Poggensee,	E-mail：ola@olasciences.com
Germany	Germany	Web：www.olasciences.com

Product Information
Applications	WB, IHC-P, IF/ICC
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:2000-10000　 IHC-P=1:50-300　 IF/ICC=1:50-300
MW (kDa)	29
Conjugate	Unconjugate
Specificity	PCNA Mouse Monoclonal Antibody detects endogenous levels of PCNA protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	PCNA; Proliferating cell nuclear antigen; Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; Pcna/cyclin; PCNAR; ATLD2
Gene Aliases	PCNA
UniProt ID	P12004
Entrez Gene ID	5111

Product Description

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Product Image Gallery

Western blot analysis of extracts on different cells, using PCNA Mouse Monoclonal Antibody (EAB10347) at 1:5000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Lane1: Hela
Lane2: Rat brain
Lane3: NIH/3T3
Lane4: 293T

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue, using PCNA Mouse Monoclonal Antibody (EAB10347) at 1:200 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol