Monoclonal Antibodies - β-tubulin Mouse Monoclonal Antibody

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β-tubulin Mouse Monoclonal Antibody

Catalog #：EAB10327

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB10327-100UL	In Stock	100ul	￡196.90
EAB10327-200UL	In Stock	200ul	￡306.90

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

Please select the country you are in to find your local distributor.

	CUlabSciences	Phone：+44 (0) 1232 330008
	Cambridge House St Thomas' Place Ely,	E-mail：sales@culabsciences.co.uk
United Kingdom	Cambridge CB27 9RD UK	Web：www.culabsciences.co.uk

Product Information
Applications	WB, IHC-P, IF/ICC
Species Reactivity	Human, Mouse, Rat, Pig, Rabbit, Dog
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:2000-10000　 IHC-P=1:50-300 IF/ICC=1:50-300
MW (kDa)	50
Conjugate	Unconjugate
Specificity	β-tubulin Mouse Monoclonal Antibody detects endogenous levels of β-tubulin protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56; Beta Tubulin; TUBB; β-Tubulin
Gene Aliases	TUBB
UniProt ID	P07437
Entrez Gene ID	203068

Product Description

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.

Product Image Gallery

Western blot analysis of extracts on different cells, using β-tubulin Mouse Monoclonal Antibody (EAB10327) at 1:5000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Lane1: A549
Lane2: A431
Lane3: HepG2
Lane4: Mouse brain

Predicted band size: 50 kDa; Observed band size: 55 kDa

Immunohistochemistry analysis of paraffin-embedded Human colorectal cance tissue, using β-tubulin Mouse Monoclonal Antibody (EAB10327) at 1:200 dilution.

Immunofluorescence analysis of Hela cell, using β-tubulin Mouse Monoclonal Antibody (EAB10327) at 1:100 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol