Monoclonal Antibodies - eNOS/NOS3 Mouse Monoclonal Antibody

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eNOS/NOS3 Mouse Monoclonal Antibody

Catalog #：EAB10273

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB10273-30UL	In Stock	30ul	$133.50
EAB10273-100UL	In Stock	100ul	$298.50
EAB10273-200UL	In Stock	200ul	$508.50

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	EbioCell	Phone：(540) 808-3925
	Imperial Business Park 4819 Emperor Boulevard,	E-mail：order@ebiocell.com
Canada	Suite 408 Durham, NC 27703, USA	Web：www.ebiocell.com

Product Information
Applications	WB, IHC-P
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:1000-5000　 IHC-P=1:100-500
MW (kDa)	133
Conjugate	Unconjugate
Specificity	eNOS/NOS3 Mouse Monoclonal Antibody detects endogenous levels of eNOS/NOS3 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Nitric oxide synthase 3; eNOS; ECNOS; NOS3
Gene Aliases	NOS3
UniProt ID	P29474
Entrez Gene ID	4846

Product Description

NOS3 also known as eNOS plays a crucial role in the cardiovascular system by synthesizing nitric oxide (NO), a signaling molecule that regulates vascular tone and blood flow. Proper functioning of NOS3 is vital for maintaining endothelial health and preventing cardiovascular diseases, as NOS3 helps mediate vasodilation and inhibit platelet aggregation. NOS3 is primarily located in endothelial cells lining blood vessels, where NOS3 activity is tightly regulated by calcium levels and calmodulin binding, ensuring NO production is responsive to physiological demands. Dysregulation of NOS3 can lead to various pathologies, including hypertension and atherosclerosis, highlighting NOS3′s importance in vascular biology and potential as a therapeutic target.

Product Image Gallery

Western blot analysis of extracts on HuvEc, Jurkat, Rat heart cells, using eNOS/NOS3 Mouse Monoclonal Antibody (EAB10273) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Predicted band size: 133 kDa; Observed band size: 130-140 kDa

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue, using eNOS/NOS3 Mouse Monoclonal Antibody (EAB10273) at 1:500 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol