Polyclonal Antibodies - Phospho-IkBε (Ser22) Rabbit Polyclonal Antibody

Phospho-IkBε (Ser22) Rabbit Polyclonal Antibody

Catalog #：EAB13137

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	EbioCell	Phone：(540) 808-3925
	Imperial Business Park 4819 Emperor Boulevard,	E-mail：order@ebiocell.com
United States	Suite 408 Durham, NC 27703, USA	Web：www.ebiocell.com

Product Information
Applications	WB, IHC-P, IF/ICC, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-300　 IF/ICC=1:50-300
MW (kDa)	53
Conjugate	Unconjugate
Specificity	Phospho-IkBε (Ser22) Rabbit Polyclonal Antibody detects endogenous levels of IkBε protein only when phosphorylated at Ser22.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	I-kappa-B-epsilon; IkB-E; ikB-epsilon; IKBE; NF-kappa-BIE; NFKBIE; Slc35b2
Gene Aliases	NFKBIE
UniProt ID	O00221
Entrez Gene ID	4794

Product Description
The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus.

Product Image Gallery
Western blot analysis of extracts on Jurkat cells treated with TNFalpha, using Phospho-IkBε (Ser22) Rabbit Polyclonal Antibody (EAB13137) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide. Predicted band size: 53 kDa; Observed band size: 38 kDa

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol