Polyclonal Antibodies - Phospho-YAP (Ser127) Rabbit Polyclonal Antibody

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Phospho-YAP (Ser127) Rabbit Polyclonal Antibody

Catalog #：EAB10211

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB10211-30UL	In Stock	30ul	€141.70
EAB10211-100UL	In Stock	100ul	€297.70
EAB10211-200UL	In Stock	200ul	€479.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	Dianost	Phone：+49 (0) 40 65472-053
	Bullenkoppel 11， 23896 Poggensee,	E-mail：ola@olasciences.com
Germany	Germany	Web：www.olasciences.com

Product Information
Applications	WB, IHC-P, IF/ICC, ELISA
Species Reactivity	Human, Mouse, Rat, Monkey
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-300　 IF/ICC=1:50-300
MW (kDa)	54
Conjugate	Unconjugate
Specificity	Phospho-YAP (Ser127) Rabbit Polyclonal Antibody detects endogenous levels of YAP protein only when phosphorylated at Ser127.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	AI325207; COB1; YAP; YAP1; YAP2; YAP65; YKI; Yorkie
Gene Aliases	YAP1
UniProt ID	P46937
Entrez Gene ID	10413

Product Description

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Image Gallery

Western blot analysis of extracts on COS7 cells treated with HU, using Phospho-YAP (Ser127) Rabbit Polyclonal Antibody (EAB10211) at 1:500 dilution. The lane on the left is blocked with the phospho peptide.

Predicted band size: 54 kDa; Observed band size: 65-78 kDa

Western blot analysis of extracts on different cells, using Phospho-YAP (Ser127) Rabbit Polyclonal Antibody (EAB10211) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Lane1: MCF7
Lane2: PC3
Lane3: Hela

Predicted band size: 54 kDa; Observed band size: 65-78 kDa

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol