Polyclonal Antibodies - Tri-Methyl-Histone H3 (Lys14) Rabbit Polyclonal Antibody

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Tri-Methyl-Histone H3 (Lys14) Rabbit Polyclonal Antibody

Catalog #：EAB10194

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB10194-30UL	In Stock	30ul	€141.70
EAB10194-100UL	In Stock	100ul	€297.70
EAB10194-200UL	In Stock	200ul	€479.70

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email us at info@ebiocell.com

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	SBT BioChem Srl	Phone：+39 05 7156 8147
	Via Ponte di Bagnolo, 811 Bologna,	E-mail：info@sbtbiochem.it
Italy	Italy	Web：www.sbtbiochem.it

Product Information
Applications	WB, IHC-P, ELISA
Species Reactivity	Human, Rat
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:100-500
MW (kDa)	15
Conjugate	Unconjugate
Specificity	Tri-Methyl-Histone H3 (Lys14) Rabbit Polyclonal Antibody detects endogenous levels of histone H3 only when tri-methylated on Lys14.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	H3 clustered histone 1; H3/A; H31; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; Histone H3; H3K14ME3
Gene Aliases	H3K14ME3
UniProt ID	P68431
Entrez Gene ID	8350

Product Description

Histone H3 is one of the four core histones (H2A, H2B, H3, and H4) essential for the formation of nucleosomes, which are fundamental units of chromatin structure in eukaryotic cells. Histone H3 is a core part of the nucleosome′s octameric structure that wraps DNA, regulating gene expression and DNA replication. Histone H3 plays a crucial role in chromatin′s structural organization, essential for DNA packaging within the nucleus. Histone H3 maintains genomic stability and facilitates transcription and repair processes. Histone H3 undergoes various post-translational modifications, including methylation and acetylation, influencing DNA and protein interactions, affecting chromatin structure and function. These modifications regulate gene expression and impact cellular differentiation and environmental response.

Product Image Gallery
Western blot analysis of extracts on Hela cells, using Tri-Methyl-Histone H3 (Lys14) Rabbit Polyclonal Antibody (EAB10194) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution. Immunohistochemical analysis of paraffin-embedded Human breast caricnoma tissue, using Tri-Methyl-Histone H3 (Lys14) Rabbit Polyclonal Antibody (EAB10194) at 1:200 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol