Polyclonal Antibodies - C/EBPα Rabbit Polyclonal Antibody

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C/EBPα Rabbit Polyclonal Antibody

Catalog #：EAB10172

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB10172-30UL	In Stock	30ul	￡97.90
EAB10172-100UL	In Stock	100ul	￡218.90
EAB10172-200UL	In Stock	200ul	￡372.90

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email us at info@ebiocell.com

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	CUlabSciences	Phone：+44 (0) 1232 330008
	Cambridge House St Thomas' Place Ely,	E-mail：sales@culabsciences.co.uk
United Kingdom	Cambridge CB27 9RD UK	Web：www.culabsciences.co.uk

Product Information
Applications	WB, IHC-P, IF, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-300　　 IF=1:50-300
MW (kDa)	38
Conjugate	Unconjugate
Specificity	C/EBPα Rabbit Polyclonal Antibody detects endogenous levels of C/EBPα protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	C/EBP-alpha; C/ebpalpha; CBF-A; CEBP; CEBPA; DBPCEP
Gene Aliases	CEBPA
UniProt ID	P49715
Entrez Gene ID	1050

Product Description

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons.

Product Image Gallery

Western blot analysis of extracts on 293 cells, using C/EBPα Rabbit Polyclonal Antibody (EAB10172) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.

Predicted band size: 38 kDa; Observed band size: 42 kDa

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue, using C/EBPα Rabbit Polyclonal Antibody (EAB10172) at 1:100 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol