Polyclonal Antibodies - Phospho-AMPKα1/2 (Thr183/172) Rabbit Polyclonal Antibody

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Phospho-AMPKα1/2 (Thr183/172) Rabbit Polyclonal Antibody

Catalog #：EAB10169

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB10169-30UL	In Stock	30ul	€141.70
EAB10169-100UL	In Stock	100ul	€297.70
EAB10169-200UL	In Stock	200ul	€479.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	Betaclonal	Phone：+34 (0)91 471 6763
	29/1 Lomonosovsky Pr,	E-mail：tech@betaclonal.com
Spain	Madrid, Spain	Web：www.betaclonal.com

Product Information
Applications	WB, IHC-P, IF/ICC, ELISA
Species Reactivity	Human, Mouse, Rat, Monkey, Pig
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-300　 IF/ICC=1:50-300
MW (kDa)	64
Conjugate	Unconjugate
Specificity	Phospho-AMPKα1/2 (Thr183/172) Rabbit Polyclonal Antibody detects endogenous levels of AMPKα1/2 only when phosphorylated at Thr183/172.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	AMPK; AMPK1; AMPKa1; AMPKalpha1; AMPKalpha1/AMPKalpha2; PRKAA1/PRKAA2; PRKAA1/2; AMPKα; AMPKα1/2; AMPKα1/AMPKα2
Gene Aliases	PRKAA1/PRKAA2
UniProt ID	Q13131, P54646
Entrez Gene ID	5562, 5563

Product Description

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Product Image Gallery

Western blot analysis of extracts on COS7 cells, using Phospho-AMPKα1/2 (Thr183/172) Rabbit Polyclonal Antibody (EAB10169) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.

Western blot analysis of extracts on MNK45, HepG2, Mouse lung, PC12 cells, using Phospho-AMPKα1/2 (Thr183/172) Rabbit Polyclonal Antibody (EAB10169) at 1:500 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue, using Phospho-AMPKα1/2 (Thr183/172) Rabbit Polyclonal Antibody (EAB10169) at 1:100 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol