Monoclonal Antibodies - Caspase-8 Mouse Monoclonal Antibody

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Caspase-8 Mouse Monoclonal Antibody

Catalog #：EAB10081

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB10081-100UL	In Stock	100ul	€115.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

Please select the country you are in to find your local distributor.

	Betaclonal	Phone：+34 (0)91 471 6763
	29/1 Lomonosovsky Pr,	E-mail：tech@betaclonal.com
Spain	Madrid, Spain	Web：www.betaclonal.com

Product Information
Applications	WB, IHC-P, IF/ICC, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:1000-5000　 IHC-P=1:100-500　 IF/ICC=1:100-500
MW (kDa)	55
Conjugate	Unconjugate
Specificity	Caspase-8 Mouse Monoclonal Antibody detects endogenous levels of Caspase-8 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Caspase 8; CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8; CASP8
Gene Aliases	CASP8
UniProt ID	Q14790
Entrez Gene ID	841

Product Description

Caspase-8 also known as MCH5, CASP8 is a member of the cysteine-aspartic acid protease (caspase) family. Caspase-8 plays a crucial role in the apoptotic signaling pathway, acting as an initiator caspase that activates downstream effector caspases through the cleavage of their inactive precursors. This process is vital for the regulation of programmed cell death, particularly in response to death receptor signaling via tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and Fas (TNFRSF6). Caspase-8 is primarily located in the cytoplasm and is recruited to the death-inducing signaling complex (DISC) upon binding to the adaptor molecule FADD, where activation occurs. The active form of caspase-8, a heterodimer composed of p18 and p10 subunits, is then released from the DISC to propagate the apoptotic signal.

Product Image Gallery

Western blot analysis of extracts on Hela, Mouse Brain, Rat Brain cells, using Caspase-8 Mouse Monoclonal Antibody (EAB10081) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Predicted band size: 55 kDa; Observed band size: 43, 57 kDa

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue, using Caspase-8 Mouse Monoclonal Antibody (EAB10081) at 1:500 dilution.

Immunofluorescence analysis of Hela cell, using Caspase-8 Mouse Monoclonal Antibody (EAB10081)(green) at 1:200 dilution(4° overnight).

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol