Monoclonal Antibodies - AMPKα1 Mouse Monoclonal Antibody

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AMPKα1 Mouse Monoclonal Antibody

Catalog #：EAB10035

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB10035-30UL	In Stock	30ul	€115.70
EAB10035-100UL	In Stock	100ul	€258.70
EAB10035-200UL	In Stock	200ul	€440.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

Please select the country you are in to find your local distributor.

	Betaclonal	Phone：+34 (0)91 471 6763
	29/1 Lomonosovsky Pr,	E-mail：tech@betaclonal.com
Spain	Madrid, Spain	Web：www.betaclonal.com

Product Information
Applications	WB, IHC-P, IF
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-300　 IF=1:50-300
MW (kDa)	64
Conjugate	Unconjugate
Specificity	AMPKα1 Mouse Monoclonal Antibody detects endogenous levels of AMPKα1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	AI194361; AI450832; AL024255; AMPK; AMPK1; AMPKa1; AMPKalpha1; C130083N04Rik; PRKAA1
Gene Aliases	PRKAA1
UniProt ID	Q13131
Entrez Gene ID	5562

Product Description

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Product Image Gallery
Western blot analysis of extracts on different cells, using AMPKα1 Mouse Monoclonal Antibody (EAB10035) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution. Lane1: Hela Lane2: 293T Lane3: NIH/3T3 Lane4: PC12 Immunohistochemical analysis of paraffin-embedded Mouse colon tissue, using AMPKα1 Mouse Monoclonal Antibody (EAB10035) at 1:200 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol