Polyclonal Antibodies - Phospho-NFkB p65 (Ser536) Rabbit Polyclonal Antibody

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Phospho-NFkB p65 (Ser536) Rabbit Polyclonal Antibody

Catalog #：EAB10019

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB10019-30UL	In Stock	30ul	€141.70
EAB10019-100UL	In Stock	100ul	€297.70
EAB10019-200UL	In Stock	200ul	€479.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

Please select the country you are in to find your local distributor.

	Betaclonal	Phone：+34 (0)91 471 6763
	29/1 Lomonosovsky Pr,	E-mail：tech@betaclonal.com
Spain	Madrid, Spain	Web：www.betaclonal.com

Product Information
Applications	WB, IP, IHC-P, IF/ICC, ELISA
Species Reactivity	Human, Mouse, Rat, Monkey
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IP=1:20-200　 IHC-P=1:50-300　 IF/ICC=1:50-300
MW (kDa)	60
Conjugate	Unconjugate
Specificity	Phospho-NFkB p65 (Ser536) Rabbit Polyclonal Antibody detects endogenous levels of NFkB p65 only when phosphorylated at Ser536.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	p65; CMCU; NFKB3; RELA; TF65; Transcription factor p65; NFκBp65; NF-κBp65; NFkB-P65; NF-kBp65
Gene Aliases	RELA
UniProt ID	Q04206
Entrez Gene ID	5970

Product Description

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Product Image Gallery

Western blot analysis of extracts on different cells, using Phospho-NFkB p65 (Ser536) Rabbit Polyclonal Antibody (EAB10019) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Lane1: HCT116
Lane2: Raw264.7
Lane3: SW480

Predicted band size: 60 kDa; Observed band size: 65 kDa

Western blot analysis of extracts on K562 cells, using Phospho-NFkB p65 (Ser536) Rabbit Polyclonal Antibody (EAB10019) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.

Predicted band size: 60 kDa; Observed band size: 65 kDa

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue, using Phospho-NFkB p65 (Ser536) Rabbit Polyclonal Antibody (EAB10019) at 1:100 dilution.

Immunofluorescence analysis of Hela cell, using Phospho-NFkB p65 (Ser536) Rabbit Polyclonal Antibody (EAB10019) at 1:200 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol