USA£º(540) 808-3925
China£º400-117-0038
Spain
Shopping Cart
Sign in \ Register
Cleaved Caspase-3 p17 (Asp175) Rabbit Polyclonal Antibody
Catalog #£ºEAB10027
  SKU-Pack Size Availability Size Price
EAB10027-30UL In Stock 30ul €141.70
EAB10027-100UL In Stock 100ul €297.70
EAB10027-200UL In Stock 200ul €479.70
If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com
Please select the country you are in to find your local distributor.
Betaclonal Phone£º+34 (0)91 471 6763
29/1 Lomonosovsky Pr, E-mail£ºtech@betaclonal.com
Spain Madrid, Spain Web£ºwww.betaclonal.com
Product Information
Applications WB, IHC-P, IF/ICC, ELISA
Species Reactivity Human, Mouse, Rat
Host / Isotype Rabbit IgG
Clonality Polyclonal
Applications Dilutions WB=1:500-2000¡¡
IHC-P=1:50-300¡¡¡¡
IF/ICC=1:50-300
MW (kDa) 32
Conjugate Unconjugate
Specificity Cleaved Caspase-3 p17 (Asp175) Rabbit Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-3 p17 protein resulting from cleavage adjacent to Asp175.
Purification Affinity purification
Concentration 1mg/ml
Format Liquid
Formulation In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping Gel Pack
Storage Store at -20¡ãC least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4¡ãC for 1-2 weeks.
Research Use For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key

WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry

Reactivity Key

H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s) AC-3; Apopain; CASP-3; CASP3; Caspase-3; CC3; CPP-32; CPP32; CPP32B; Lice; mldy; SCA-1; Yama
Gene Aliases CASP3
UniProt ID

P42574

Entrez Gene ID

836

Product Description

Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.

Product Image Gallery
Specific Protocols
>> Western Blotting Protocol >> Immunoprecipitation Protocol
>> Immunohistochemistry Protocol >> Immunofluorescence Protocol
>> Immunocytochemistry Protocol >> Flow Cytometry Protocol
>> ChIP Protocol >> ELISA Protocol
>> HPLC Protocol >> PCR Protocol
For Research Use Only, Not For Diagnostic Or Therapeutic Procedures.
Copyright 2015 EbioCell, Inc. All Rights Reserved. ¡¡±¸°¸ºÅ£º½òICP±¸2022005142ºÅ-1