B-27 Supplement is a serum-free supplement which is used for the growth and short or long term excitation of hippocampal neurons and other neuronal cells from central nervous system. B-27 supplement is a 50X liquid that is suitable for the cultivation of most neuronal cells. This product does not contain 5 types of antioxidants including Vitamin E, Vitamin E acetate, superoxide dismutase, catalase and glutathione to prevent the interference of antioxidants during experiments.