Protein Substrates - SM-164 (CAS: 957135-43-2)

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SM-164 (CAS: 957135-43-2)

Catalog #：EBC51022

Datasheet

SM-164 (CAS: 957135-43-2)

SKU-Pack Size	Availability	Size	Price
EBC51022-1ML	In Stock	1mL(10mM in DMSO)	€778.70
EBC51037-2MG	In Stock	2mg	€310.70
EBC51022-5MG	In Stock	5mg	€427.70
EBC51022-10MG	In Stock	10mg	€544.70
EBC51022-25MG	In Stock	25mg	€1038.70

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Product Information

Synonym(s)

SM 164, SM164

Chemical Name

(S,3S,3'S,6S,6'S,10aS,10a'S)-N,N'-((1S,1'S)-(1,1'-(4,4'-(1,4-phenylene)bis(butane-4,1-diyl))bis(1H-1,2,3-triazole-4,1-diyl))bis(phenylmethylene))bis(6-((S)-2-(2-methylamino)propanamido)-5-oxodecahydropyrrolo[1,2-a]azocine-3-carboxamide)

Application

SM-164 is a bivalent mimetic of Smac

CAS Number

957135-43-2

Purity

≥99.0%

Molecular Weight

1121.42

Molecular Formula

C62H84N14O6

SMILES

O=C([C@H]1N(C([C@@H](NC([C@H](C)NC)=O)CCCC2)=O)[C@]2([H])CC1)N[C@H](C3=CN(CCCCC4=CC=C(CCCCN5C=C([C@H](NC([C@@H]6CC[C@](CCCC[C@@H]7NC([C@@H](NC)C)=O)([H])N6C7=O)=O)C8=CC=CC=C8)N=N5)C=C4)N=N3)C9=CC=CC=C9

Target & IC50

XIAP: IC50 = 0.56nM(Ki)
cIAP-1: IC50 = 0.31nM(Ki)
cIAP-2: IC50 = 1.1nM(Ki)

Solubility

DMSO: 5.2 mg/mL (4.64 mM)

Preparing Stock Solutions

ConcentrationSolventMass	1 mg	5 mg	10 mg
1 mM	0.8917 ml	4.4586 ml	8.9173 ml
5 mM	0.1783 ml	0.8917 ml	1.7835 ml
10 mM	0.0892 ml	0.4459 ml	0.8917 ml
50 mM	0.0178 ml	0.0892 ml	0.1783 ml

Shipping

Gel Pack

Storage

Store at -20°C

Research Use

For Research Use Only. Not Intended for Diagnostic Or Therapeutic Use.

Product Description

SM-164 is developed as an anticancer agent. It plays its antitumor roles through inducing degradation of cellular inhibitor of apoptosis protein (cIAP)-1/2, antagonizing X-linked inhibitor of apoptosis protein (XIAP) and inducing TNFα–dependent apoptosis in tumor cells. SM-164 is a bivalent mimetic containing two SM-122 analogues. It binds to cIAP-1 protein containing bothBIR2 and BIR3 domains, cIAP-2 BIR3 protein and XIAP protein containing both BIR2 and BIR3 domains with Ki values of 0.31 nM, 1.1 nM and 0.56 nM, respectively. In tumor cells, treatment of SM-164 significantly reduced cIAP-1 level to undetectable levels (1nM, 60min), effectively antagonized cellular XIAP and enhanced TNFα secretion. In the MDA-MB-231 xenograft model, administration of SM-164 at dose of 5mg/kg markedly decreased cIAP-1 level within 1 hour and activated caspase-8, caspase-9 and caspase-3 at 3 hour 1.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol