Phleomycin is a glycopeptide antibiotic of the bleomycin family, isolated from a mutant strain ofStreptomyces verticillus. It binds and intercalates DNA thus destroying the integrity of the double helix. Phleomycin is active against most bacteria, filamentous fungi, yeast, plant and animal cells.

Phleomycin is used at a concentration of 10 µg/ml for yeasts and 25-150 µg/ml for filamentous fungi.