Monoclonal Antibodies - Oct-4 Mouse Monoclonal Antibody

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Oct-4 Mouse Monoclonal Antibody

Catalog #：EAB22621

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB22621-100UL	In Stock	100ul	€297.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	SBT BioChem Srl	Phone：+39 05 7156 8147
	Via Ponte di Bagnolo, 811 Bologna,	E-mail：info@sbtbiochem.it
Italy	Italy	Web：www.sbtbiochem.it

Product Information
Applications	WB, IF/ICC, FC
Species Reactivity	Human
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:1000-5000　 IF/ICC=1:100-500　 FC=1:50-200
MW (kDa)	39
Conjugate	Unconjugate
Specificity	Oct-4 Mouse Monoclonal Antibody detects endogenous levels of Oct-4 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	OCT3; OCT4; OTF3; OTF-3; NF-A3; NFA3; Oct-3; Oct-3/4; Oct-4; OCT3; Oct3/4; OCT4; OTF-3; Otf-4; OTF3; Otf3-rs7; Otf3g; POU5F1
Gene Aliases	POU5F1
UniProt ID	Q01860
Entrez Gene ID	5460

Product Description

POU5F1 (POU domain, class 5, transcription factor 1), also known as octamer-binding transcription factor-3 (Oct-3, Otf-3), octamer-binding transcription factor-4 (Oct-4, Otf-4) and Oct-3/4, modulates embryonic stem (ES) cell populations by influencing lineage commitment. Oct-3/4 sustains stem-cell self-renewal and differentiation pathways. Transcription factors containing the POU homeodomain regulate tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Oct-3/4 is capable of inducing rapid proliferation and tumorigenic properties of ES cells through activation of the UTF1 gene. In humans, two Oct-3/4 isoforms contribute to influencing the undifferentiated phenotype of ES cells. Oct-3/4 pseudogenes localizing to human chromosomes 10 and 8 are reported to be transcribed in certain cancer cell lines and tissues.

Product Image Gallery
Western blot analysis of extracts on different cells, using Oct-4 Mouse Monoclonal Antibody (EAB22621) at 1:5000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution. Lane1: SW1990 Lane2: F9 Predicted band size: 39 kDa; Observed band size: 50 kDa

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol