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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, FC, ELISA | 25 | Human |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:100-500 FC=1:50-200 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | HPRT Mouse Monoclonal Antibody detects endogenous levels of HPRT protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HPRT(Hypoxanthine phosphoribosyltransferase) is a crucial cytoplasmic protein composed and belongs to the purine/pyrimidine phosphoribosyltransferase family. HPRT plays a vital role in purine metabolism as a purine salvage enzyme. HPRT catalyzes the conversion of hypoxanthine and guanine into their respective mononucleotides, inosine monophosphate and guanosine monophosphate, thereby facilitating the recycling of purines, which are essential for DNA and RNA synthesis. HPRT exists as a homotetramer and requires two magnesium ions as cofactors for enzymatic activity. Deficiencies in HPRT can lead to serious metabolic disorders, including gout and Lesch-Nyhan syndrome, highlighting HPRT′s importance in maintaining purine homeostasis. |
