| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | IHC-P, IF/ICC, ELISA | 124 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-PLD1 (Ser561) Rabbit Polyclonal Antibody detects endogenous levels of PLD1 protein only when phosphorylated at Ser561. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PLD1 also known as PC-PLD1 is a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. PLD1 plays a crucial role in cellular signaling and membrane dynamics, as PLD1 is involved in the hydrolysis of phosphatidylcholine to produce phosphatidic acid, a key lipid mediator that influences various cellular processes, including cell growth, migration, and differentiation. PLD1 is primarily localized to secretory granules, where PLD1 participates in the regulation of macrophage phagocytosis and is activated by G protein-coupled and receptor tyrosine kinase signaling pathways. Notably, PLD1′s activity is essential for the stimulation of downstream signaling events, such as ERK phosphorylation and IL-8 secretion in bronchial epithelial cells, highlighting PLD1′s importance in inflammatory responses. |
| Product Image Gallery | |
 Immunohistochemistry analysis of paraffin-embedded Human brain tissue, using Phospho-PLD1 (Ser561) Rabbit Polyclonal Antibody (EAB14205) at 1:100 dilution.
 Immunofluorescence analysis of HepG2 cell, using Phospho-PLD1 (Ser561) Rabbit Polyclonal Antibody (EAB14205) at 1:100 dilution.
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