| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 309 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | VWF Rabbit Monoclonal Antibody detects endogenous levels of VWF protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
VWF(von Willebrand factor) is a vital multimeric glycoprotein essential for hemostasis, synthesized primarily in endothelial cells and stored in Weibel-Palade bodies, as well as in platelets where VWF resides in alpha granules. Upon vascular injury, VWF is rapidly secreted into the plasma, where VWF plays a critical role in blood coagulation by mediating platelet adhesion and aggregation through interaction with glycoprotein Ib (GPIb) receptors on platelets and binding to exposed collagen in the subendothelial matrix. This interaction initiates the formation of a stable blood clot, preventing excessive bleeding. Additionally, VWF serves as a carrier protein for Factor VIII, a crucial cofactor in the coagulation cascade, protecting Factor VIII from proteolytic degradation and ensuring availability during the clotting process. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2, Rat liver cells, using VWF Rabbit Monoclonal Antibody (EAB22102) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 309 kDa; Observed band size: 280 kDa |
